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optimized single-step optical clearing method  (Optimus Corp)

 
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    Structured Review

    Optimus Corp optimized single-step optical clearing method
    Optimized Single Step Optical Clearing Method, supplied by Optimus Corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clearing+method/optimized+single+step+optical+clearing+method++optimus+/pm40054059-170-24-28
    Average 90 stars, based on 1 article reviews
    optimized single-step optical clearing method - by Bioz Stars, 2026-10
    90/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N-terminal cysteine using de novo fluorophore generation.
    Article Snippet: Our research group developed a novel fluorescence staining strategy based on the DNFC targeting N-Cys in proteins.. By treating biological samples with non-fluorogenic citrate and coupling reagents, we achieved strong cyan fluorescence, enabling effective visualization of N-Cys proteins in cells and tissues.. The DNFC reaction occurs specifically on N-Cys residues, making it highly ideal for monitoring protein processing events, particularly within the Arg/N-degron pathway.

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N -terminal cysteine using de novo fluorophore generation
    Article Snippet: However, for rapid 3D imaging with light sheet fluorescence microscopy (LSFM), we rather integrated the DNFC staining with an aqueous-based fast tissue-clearing method, optimized single-step optical clearing method (OptiMus) [ ].

    Fluorescence:

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N-terminal cysteine using de novo fluorophore generation.
    Article Snippet: Our research group developed a novel fluorescence staining strategy based on the DNFC targeting N-Cys in proteins.. By treating biological samples with non-fluorogenic citrate and coupling reagents, we achieved strong cyan fluorescence, enabling effective visualization of N-Cys proteins in cells and tissues.. The DNFC reaction occurs specifically on N-Cys residues, making it highly ideal for monitoring protein processing events, particularly within the Arg/N-degron pathway.

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N -terminal cysteine using de novo fluorophore generation
    Article Snippet: However, for rapid 3D imaging with light sheet fluorescence microscopy (LSFM), we rather integrated the DNFC staining with an aqueous-based fast tissue-clearing method, optimized single-step optical clearing method (OptiMus) [ ].

    Microscopy:

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N-terminal cysteine using de novo fluorophore generation.
    Article Snippet: Our research group developed a novel fluorescence staining strategy based on the DNFC targeting N-Cys in proteins.. By treating biological samples with non-fluorogenic citrate and coupling reagents, we achieved strong cyan fluorescence, enabling effective visualization of N-Cys proteins in cells and tissues.. The DNFC reaction occurs specifically on N-Cys residues, making it highly ideal for monitoring protein processing events, particularly within the Arg/N-degron pathway.

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N -terminal cysteine using de novo fluorophore generation
    Article Snippet: However, for rapid 3D imaging with light sheet fluorescence microscopy (LSFM), we rather integrated the DNFC staining with an aqueous-based fast tissue-clearing method, optimized single-step optical clearing method (OptiMus) [ ].

    Staining:

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N-terminal cysteine using de novo fluorophore generation.
    Article Snippet: Our research group developed a novel fluorescence staining strategy based on the DNFC targeting N-Cys in proteins.. By treating biological samples with non-fluorogenic citrate and coupling reagents, we achieved strong cyan fluorescence, enabling effective visualization of N-Cys proteins in cells and tissues.. The DNFC reaction occurs specifically on N-Cys residues, making it highly ideal for monitoring protein processing events, particularly within the Arg/N-degron pathway.

    Article Title: 3D Histology visualizing hypoxia-induced upregulation of N -terminal cysteine using de novo fluorophore generation
    Article Snippet: However, for rapid 3D imaging with light sheet fluorescence microscopy (LSFM), we rather integrated the DNFC staining with an aqueous-based fast tissue-clearing method, optimized single-step optical clearing method (OptiMus) [ ].



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

    doi: 10.1016/j.xpro.2022.101703

    Figure Lengend Snippet:

    Article Snippet: The described protocol is an adaptation of the whole-mount immunostaining protocol developed by ( ) and the RapiClear 1.47 clearing method developed by the SunJin Lab ( ; ).

    Techniques: Recombinant, Fluorsave, Software, Microscopy